Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Dublin, Ireland.
ACS Nano. 2011 Sep 27;5(9):7503-9. doi: 10.1021/nn202458g. Epub 2011 Aug 26.
The importance of the protein corona formed around nanoparticles upon entering a biological fluid has recently been highlighted. This corona is, when sufficiently long-lived, thought to govern the particles' biological fate. However, even this long-lived "hard" corona evolves and re-equilibrates as particles pass from one biological fluid to another, and may be an important feature for long-term fate. Here we show the evolution of the protein corona as a result of transfer of nanoparticles from one biological fluid (plasma) into another (cytosolic fluid), a simple illustrative model for the uptake of nanoparticles into cells. While no direct comparison can be made to what would happen in, for example, the uptake pathway, the results confirm that significant evolution of the corona occurs in the second biological solution, but that the final corona contains a "fingerprint" of its history. This could be evolved to map the transport pathways utilized by nanoparticles, and eventually to predict nanoparticle fate and behavior.
近年来，人们越来越重视纳米粒子进入生物流体后形成的蛋白质冠。当这种冠足够稳定时，它被认为会影响粒子的生物学命运。然而，即使是这种“硬”的长寿命蛋白质冠，也会随着粒子从一种生物流体转移到另一种生物流体而发生演变和重新平衡，并且可能是长期命运的一个重要特征。在这里，我们展示了纳米粒子从一种生物流体（血浆）转移到另一种生物流体（胞质溶胶）时蛋白质冠的演变，这是纳米粒子进入细胞的摄取途径的一个简单说明性模型。虽然不能直接与例如摄取途径中的情况进行比较，但结果证实，在第二个生物溶液中，蛋白质冠会发生显著的演变，但最终的蛋白质冠包含其历史的“指纹”。这可以用来绘制纳米粒子所利用的运输途径，并最终预测纳米粒子的命运和行为。
