视网膜脱离与复位猫模型的建立

The Development of a Cat Model of Retinal Detachment and Re-attachment.

作者信息

Wassmer Sarah, Leonard Brian C, Coupland Stuart G, Baker Adam, Hamilton John, Torlone Renée, Zacks David N, Tsilfidis Catherine

机构信息

Ottawa Hospital Research Institute, Regenerative Medicine, K1H 8L6, Ottawa, ON, Canada.

Department of Cellular and Molecular Medicine, University of Ottawa, K1H 8M5, Ottawa, ON, Canada.

出版信息

Adv Exp Med Biol. 2016;854:315-21. doi: 10.1007/978-3-319-17121-0_42.

DOI:10.1007/978-3-319-17121-0_42

PMID:26427427

Abstract

We present an optimized surgical technique for feline retinal detachment which allows for natural re-attachment, reduces retinal scarring and vitreal bands, and allows central placement of the detachment in close proximity to the optic nerve. This enables imaging via Optical Coherence Tomography (OCT) and multifocal electroretinography (mfERG) analysis. Ideal detachment conditions involve a lensectomy followed by a three-port pars plana vitrectomy. A 16-20 % retinal detachment is induced by injecting 8 % C3F8 gas into the subretinal space in the central retina with a 42G cannula. The retinal detachment resolves approximately 6 weeks post-surgery. Imaging is enhanced by using a 7.5 and 20 diopter lens for OCT and mfERG fundus imaging, respectively, to compensate for the removed lens.

摘要

我们提出了一种针对猫视网膜脱离的优化手术技术，该技术可实现自然复位，减少视网膜瘢痕和玻璃体条索，并使脱离部位靠近视神经中心放置。这使得能够通过光学相干断层扫描（OCT）和多焦视网膜电图（mfERG）分析进行成像。理想的脱离条件包括晶状体切除术，然后进行三通道扁平部玻璃体切除术。通过用42G套管将8%的C3F8气体注入中央视网膜的视网膜下间隙，诱导16 - 20%的视网膜脱离。视网膜脱离在手术后约6周消退。分别使用7.5屈光度和20屈光度的透镜进行OCT和mfERG眼底成像，以补偿移除的晶状体，从而增强成像效果。