Key Lab of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Haidian District, Beijing, China.
Anaerobe. 2011 Oct;17(5):226-31. doi: 10.1016/j.anaerobe.2011.07.010. Epub 2011 Aug 24.
The aim of the study was to purify the exopolysaccharides (EPS) produced from Bifidobacterium animalis RH, which was isolated from the feces of Bama centenarians in Guangxi of China, and evaluate their antioxidant activities in vitro and in vivo. 2 fractions, a neutral EPS fraction (EPSa) and an acidic EPS fraction (EPSb), were obtained and compared for antioxidative activity. In vitro, they both showed remarkable inhibition effect on lipid peroxidation and strong DPPH radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, in which the last two were measured by the electron spin resonance (ESR). In vivo, EPSa and EPSb were orally administrated for 30 days in a d-galactose induced aged mice model. As results, they both could significantly increase the activities of SOD, CAT and total antioxidant capacity (TAOC) in serums and glutathione GST in livers. They also could inhibit significantly the formation of MDA in serums and livers, and reduce the activity of MAO and lipofuscin accumulation in mice brain. Moreover, EPSb exhibited much higher antioxidant activities than EPSa in vitro and in vivo. The results suggested that EPS fractions of Bifidobacterium animalis RH had direct and potent antioxidant activities.
本研究旨在从中国广西巴马长寿老人粪便中分离出的双歧杆菌 RH 中提取并纯化其胞外多糖（EPS），并评估其在体外和体内的抗氧化活性。得到了 2 种馏分，一种中性 EPS 馏分（EPSa）和一种酸性 EPS 馏分（EPSb），并对其抗氧化活性进行了比较。体外实验表明，它们均对脂质过氧化具有显著的抑制作用，且对 DPPH 自由基、羟自由基和超氧自由基具有很强的清除能力，后两者通过电子自旋共振（ESR）进行测量。体内实验中，EPSa 和 EPSb 连续 30 天经口给予半乳糖诱导的衰老小鼠模型。结果表明，它们均能显著提高血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总抗氧化能力（TAOC）以及肝脏谷胱甘肽 S-转移酶（GST）的活性。它们还能显著抑制血清和肝脏丙二醛（MDA）的形成，降低小鼠脑中单胺氧化酶（MAO）的活性和脂褐素的积累。此外，EPSb 在体外和体内均表现出比 EPSa 更高的抗氧化活性。这些结果表明，双歧杆菌 RH 的 EPS 馏分具有直接而有效的抗氧化活性。
