Department of Food Science and Technology, Jinling College, Nanjing Normal University, Nanjing 210097, China.
Food Chem. 2013 May 1;138(1):84-9. doi: 10.1016/j.foodchem.2012.10.029. Epub 2012 Nov 2.
Exopolysaccharide (EPS) was isolated and purified from Lactococcus lactis subsp. Lactis culture broth. Selenium chloride oxide (SeCl(2)O) was added to the EPS to synthesize selenium-exopolysaccharide (Se-EPS). The in vitro and in vivo antioxidant and in vivo immunomodulatory activity of EPS and Se-EPS were compared. EPS and Se-EPS scavenged superoxide anions and hydroxyl radicals. They also increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, while decreasing malondialdehyde (MDA) levels in serum and in the livers of mice. Se-EPS showed stronger in vitro and in vivo antioxidant activity than were shown by EPS. The in vivo immunoenhancement activity of EPS and Se-EPS induced by cyclophosphamide (CY) treatment in immunosuppressed mice was researched. EPS and Se-EPS treatments increased macrophage phagocytosis, spleen and thymus indices and haemolytic complement activity (HC(50)). Se-EPS showed stronger immunomodulatory activity than did EPS.
从乳球菌乳亚种培养肉汤中分离和纯化胞外多糖（EPS）。向 EPS 中加入亚硒酸氯（SeCl(2)O）以合成硒-胞外多糖（Se-EPS）。比较了 EPS 和 Se-EPS 的体外和体内抗氧化和体内免疫调节活性。EPS 和 Se-EPS 清除超氧阴离子和羟自由基。它们还增加了过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性，同时降低了血清和小鼠肝脏中丙二醛（MDA）的水平。Se-EPS 表现出比 EPS 更强的体外和体内抗氧化活性。研究了环磷酰胺（CY）处理免疫抑制小鼠时 EPS 和 Se-EPS 对体内免疫增强活性的影响。EPS 和 Se-EPS 处理增加了巨噬细胞吞噬作用、脾脏和胸腺指数以及溶血补体活性（HC(50)）。Se-EPS 表现出比 EPS 更强的免疫调节活性。
