Departamentos de Inmunología y, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico.
J Proteomics. 2011 Nov 18;74(12):2959-71. doi: 10.1016/j.jprot.2011.07.020. Epub 2011 Aug 19.
Brucella abortus is an alpha-2 proteobacteria with a type IV secretion system (T4SS) known as virB, which is necessary to gain virulence by building up a replicative vacuole associated with the endoplasmic reticulum of the host cell. A virB T4SS mutant of the B. abortus 2308 strain and its wild-type strain were grown in acid medium in order to obtain and analyze their proteomes, looking for putative proteins that may serve as T4SS substrates and those that may be subjected to T4SS regulation. A total of 47 overexpressed and 22 underexpressed proteins from the virB T4SS mutant strain were selected and sequenced. Some of the 69 analyzed proteins have not been described before either as over or under-expressed in relation to a virB T4SS mutation, whereas some of them have been already described by other groups as potentially important secretory proteins in other Brucella species. An important number of the proteins identified are outer membrane and periplasmic space protein, which makes them become particularly important new T4SS-related candidate proteins.
流产布鲁氏菌是一种α-2 变形菌，具有一种称为 virB 的 IV 型分泌系统 (T4SS)，该系统对于通过建立与宿主细胞内质网相关的复制空泡来获得毒力是必要的。为了获得和分析其蛋白质组，我们在酸性培养基中培养了流产布鲁氏菌 2308 菌株的 virB T4SS 突变体及其野生型菌株，寻找可能作为 T4SS 底物的假定蛋白和可能受到 T4SS 调节的蛋白。从 virB T4SS 突变体菌株中选择并测序了 47 种过度表达和 22 种表达不足的蛋白。分析的 69 种蛋白中有一些以前没有被描述为与 virB T4SS 突变相关的过度或表达不足，而其中一些已经被其他研究小组描述为其他布鲁氏菌物种中潜在重要的分泌蛋白。鉴定出的蛋白中有相当数量是外膜和周质空间蛋白，这使它们成为特别重要的新的 T4SS 相关候选蛋白。
