Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nanomedicine. 2012 Jul;8(5):599-608. doi: 10.1016/j.nano.2011.08.005. Epub 2011 Sep 1.
Cerium oxide nanoparticles (nanoceria) are engineered nanoparticles whose versatility is due to their unique redox properties. We and others have demonstrated that naked nanoceria can act as antioxidants to protect cells against oxidative damage. Although the redox properties may be beneficial, the genome-wide effects of nanoceria on gene transcription and associated biological processes remain elusive. Here we applied a functional genomic approach to examine the genome-wide effects of nanoceria on global gene transcription and cellular functions in mouse neuronal cells. Importantly, we demonstrated that nanoceria induced chemical- and size-specific changes in the murine neuronal cell transcriptome. The nanoceria contributed more than 83% of the population of uniquely altered genes and were associated with a unique spectrum of genes related to neurological disease, cell cycle control, and growth. These observations suggest that an in-depth assessment of potential health effects of naked nanoceria and other naked nanoparticles is both necessary and imminent.
Cerium oxide nanoparticles are important antioxidants, with potential applications in neurodegenerative conditions. This team of investigators demonstrated the genomic effects of nanoceria, showing that it induced chemical- and size-specific changes in the murine neuronal cell transcriptome.
氧化铈纳米粒子（纳米氧化铈）是一种工程纳米粒子，其多功能性归因于其独特的氧化还原特性。我们和其他人已经证明，裸露的纳米氧化铈可以作为抗氧化剂，保护细胞免受氧化损伤。尽管氧化还原特性可能是有益的，但纳米氧化铈对基因转录和相关生物过程的全基因组影响仍然难以捉摸。在这里，我们应用功能基因组学方法来研究纳米氧化铈对小鼠神经元细胞中全局基因转录和细胞功能的全基因组影响。重要的是，我们证明了纳米氧化铈诱导了鼠神经元细胞转录组中化学和大小特异性的变化。纳米氧化铈贡献了超过 83%的独特改变基因的群体，与与神经疾病、细胞周期控制和生长相关的独特基因谱相关。这些观察结果表明，对裸露纳米氧化铈和其他裸露纳米粒子的潜在健康影响进行深入评估不仅是必要的，而且是迫在眉睫的。
氧化铈纳米粒子是重要的抗氧化剂，具有在神经退行性疾病条件下的潜在应用。该研究小组的研究人员展示了纳米氧化铈的基因组效应，表明它诱导了鼠神经元细胞转录组中化学和大小特异性的变化。
