National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, P.R. China.
Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, P.R.China.
Proteome Sci. 2011 Sep 7;9:52. doi: 10.1186/1477-5956-9-52.
Phytophthora sojae causes soybean root and stem rot, resulting in an annual loss of 1-2 billion US dollars in soybean production worldwide. A proteomic technique was used to determine the effects on soybean hypocotyls of infection with P. sojae.
In the present study, 46 differentially expressed proteins were identified in soybean hypocotyls infected with P. sojae, using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight (MALDI-TOF/TOF). The expression levels of 26 proteins were significantly affected at various time points in the tolerant soybean line, Yudou25, (12 up-regulated and 14 down-regulated). In contrast, in the sensitive soybean line, NG6255, only 20 proteins were significantly affected (11 up-regulated and 9 down-regulated). Among these proteins, 26% were related to energy regulation, 15% to protein destination and storage, 11% to defense against disease, 11% to metabolism, 9% to protein synthesis, 4% to secondary metabolism, and 24% were of unknown function.
Our study provides important information on the use of proteomic methods for studying protein regulation during plant-oomycete interactions.
大豆疫霉引起大豆根腐和茎腐病，导致全球每年损失 10 亿至 20 亿美元的大豆产量。本研究采用蛋白质组学技术研究大豆下胚轴感染大豆疫霉的影响。
本研究采用二维电泳和基质辅助激光解吸/电离串联飞行时间（MALDI-TOF/TOF）技术，鉴定了感染大豆疫霉的大豆下胚轴中 46 种差异表达蛋白。在耐病品种豫豆 25 中，在不同时间点，有 26 种蛋白的表达水平受到显著影响（12 种上调，14 种下调）。相比之下，在感病品种 NG6255 中，只有 20 种蛋白的表达水平受到显著影响（11 种上调，9 种下调）。这些蛋白中，26%与能量调控有关，15%与蛋白定位和储存有关，11%与抗病性有关，11%与代谢有关，9%与蛋白合成有关，4%与次生代谢有关，24%的蛋白功能未知。
本研究为利用蛋白质组学方法研究植物-卵菌互作过程中的蛋白调控提供了重要信息。
