采用抗甲型肝炎病毒IgM直接放射免疫测定法（HAVAB-M）诊断急性甲型肝炎。

Diagnosis of acute hepatitis A by HAVAB-M, a direct radioimmunoassay for IgM anti-HAV.

作者信息

Decker R H, Kosakowski S M, Vanderbilt A S, Ling C M, Chairez R, Overby L R

出版信息

Am J Clin Pathol. 1981 Aug;76(2):140-7. doi: 10.1093/ajcp/76.2.140.

DOI:10.1093/ajcp/76.2.140

Abstract

A three-step solid-phase radioimmunoassay, HAVAB-M, was developed for use in clinical labs as an aid to diagnosing hepatitis A. Polystyrene beads were coated with anti-human IgM (mu-chain specific) and were incubated successively with serum specimen, HAV, and anti-HAV 125I. HAVAB-M was used to assay sera from patients with hepatitis A and was found to have high sensitivity for the IgM antibody to HAV. The antibody was detectable within a few days of onset of symptoms of hepatitis, and it reached maximum concentrations within one to three weeks. The test was designed so that most patients' sera would become negative approximately three months after onset. HAVAB-M was shown to be specific for IgM antibody, with virtually no detection of IgG anti-HAV. The test showed no interference fro rheumatoid factor and no cross-reactivity with sera from patients with hepatitis B or other infectious diseases.

摘要

一种三步固相放射免疫测定法，即HAVAB-M，被开发用于临床实验室，以辅助诊断甲型肝炎。聚苯乙烯珠粒用抗人IgM（μ链特异性）包被，并依次与血清标本、甲型肝炎病毒（HAV）和抗HAV 125I孵育。HAVAB-M用于检测甲型肝炎患者的血清，发现其对甲型肝炎病毒IgM抗体具有高敏感性。该抗体在肝炎症状出现后的几天内即可检测到，并在1至3周内达到最高浓度。该检测的设计使得大多数患者的血清在发病后约三个月会转为阴性。HAVAB-M显示对IgM抗体具有特异性，几乎检测不到IgG抗HAV。该检测未显示类风湿因子的干扰，且与乙型肝炎患者或其他传染病患者的血清无交叉反应。