Cardiovascular Research Institute and Department of Biochemistry, University of California San Francisco, San Francisco, California, USA.
Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
能够将精确的输入应用于活细胞中的信号分子，对于探究和理解复杂的细胞信号系统将具有变革性意义。在这里，我们报告了一种“光遗传学”方法，该方法使用光门控蛋白-蛋白相互作用的反馈控制来施加定制的信号输入。我们将该策略应用于扰动蛋白质定位和磷酸肌醇 3-激酶活性，生成时变信号并对其进行缓冲以抵抗细胞间变异性或途径活性变化的影响。
