Suzuki Hidenori, Taguchi Toshihiko, Kato Yoshihiko, Kanchiku Tsukasa, Imagama Takashi, Yara Takahiro, Moriya Atsushi, Muramatsu Keiichi, Tanaka Hiroshi, Gondo Toshikazu
Department of Orthopedic Surgery, Yamaguchi University, School of Medicine, 1-1-1 Minami-kogushi, Ube, Yamaguchi 755-8505, Japan.
Med Mol Morphol. 2011 Sep;44(3):131-8. doi: 10.1007/s00795-010-0519-y. Epub 2011 Sep 16.
Previous studies have revealed that cell therapy using bone marrow stromal cells (BMSCs) could promote motor functional recovery in animals with spinal cord injury (SCI). We describe here the development of cell biology technique and the experimental study of regeneration in SCI. The aim of this study was to investigate the potential for neurological recovery by transplantation neurospheres (NS) derived from BMSCs into thoracic SCI. Adult Fisher rats were used: 45 were subjected to complete thoracic SCI performed by the balloon compression method. BMSCs were cultured in vitro to obtain NS. Seven days after thoracic SCI, groups of 15 rats each received transplants of BMSCs-NS (group A), BMSCs (group B), or injection of medium only (group C) into the SCI lesion. Rats from each group were evaluated and compared longitudinally for motor function recovery. The spinal cords (SC) of injured rats were harvested at day 21 or day 42 and prepared for histological analysis. Five weeks after transplantation, many neuronal or axonal sproutings were observed and replaced by host cells in the SCI lesion of group A. Also, transplanted BMSCs-NS expressed neuronal lineage markers. Transplanted rats could walk with weight bearing and showed recovered motor evoked potentials (MEPs).
先前的研究表明，使用骨髓基质细胞（BMSCs）进行细胞治疗可促进脊髓损伤（SCI）动物的运动功能恢复。我们在此描述细胞生物学技术的发展以及SCI再生的实验研究。本研究的目的是探讨将源自BMSCs的神经球（NS）移植到胸段SCI中实现神经恢复的潜力。使用成年Fisher大鼠：45只通过球囊压迫法进行完全性胸段SCI。将BMSCs体外培养以获得NS。胸段SCI后7天，每组15只大鼠分别接受将BMSCs-NS（A组）、BMSCs（B组）或仅注射培养基（C组）移植到SCI损伤部位。对每组大鼠进行纵向评估并比较其运动功能恢复情况。在第21天或第42天收获受伤大鼠的脊髓（SC）并准备进行组织学分析。移植后五周，在A组SCI损伤部位观察到许多神经元或轴突发芽，并被宿主细胞替代。此外，移植的BMSCs-NS表达神经元谱系标记物。移植后的大鼠能够负重行走并显示出运动诱发电位（MEPs）恢复。
