Suzuki Hidenori, Kanchiku Tsukasa, Imajo Yasuaki, Yoshida Yuichiro, Nishida Norihiro, Gondo Toshikazu, Yoshii Satoru, Taguchi Toshihiko
Department of Orthopedic Surgery, Graduate School of Medicine, Yamaguchi University, 1-1-1 Minami-kogushi, Ube, Yamaguchi, 755-8505, Japan.
Department of Surgical Pathology, Fujisawa City Hospital, Fujisawa, Japan.
Med Mol Morphol. 2015 Dec;48(4):214-24. doi: 10.1007/s00795-015-0104-5. Epub 2015 May 16.
Traumatically injured spinal cord (SC) displays structural damage that includes discontinuity of long tracts and cavitations. Axonal regrowth beyond the lesion is necessary to achieve functional recovery following SC injury. We report here the development of an artificial collagen-filament (CF) scaffold to replace the SC in 8-week-old female Fisher rats. Axonal sprouting and regrowth was very rapid following grafting of the CF. One week after implantation, the scaffold was filled with cells of host origin and with regenerated axons. Histological examination of SC adjacent to the scaffold showed little cavity formation or fibrous scarring. Eight weeks after implantation, myelinated nerve fibers were found in the scaffold and 10-25 % of rubrospinal tracts were repaired. Four to six weeks after transplantation, motor evoked potentials were recorded in CF-grafted rats but were not detectable in non-grafted rats. Electrophysiological and histological examinations revealed the grafted CF was likely to function as a nerve tract. In addition, these results suggest that collagen fibers may provide a permissive microenvironment for the elongation of SC axons and to support the process of spinal cord regeneration.
创伤性脊髓损伤会出现包括长束中断和空洞形成在内的结构损伤。脊髓损伤后，损伤部位以外的轴突再生对于实现功能恢复至关重要。我们在此报告了一种人工胶原纤维（CF）支架的研发情况，该支架用于替代8周龄雌性Fisher大鼠的脊髓。植入CF后，轴突发芽和再生非常迅速。植入一周后，支架内充满了宿主来源的细胞和再生轴突。对支架附近脊髓的组织学检查显示几乎没有空洞形成或纤维瘢痕。植入八周后，在支架内发现了有髓神经纤维，并且10%-25%的红核脊髓束得到修复。移植后四到六周，在植入CF的大鼠中记录到了运动诱发电位，但在未植入的大鼠中未检测到。电生理和组织学检查表明，植入的CF可能起到了神经束的作用。此外，这些结果表明胶原纤维可能为脊髓轴突的延伸提供了一个有利的微环境，并支持脊髓再生过程。
