传染性单核细胞增多症来源的人类淋巴细胞系中细胞内环状爱泼斯坦-巴尔病毒DNA分子的大小
Size of the intracellular circular Epstein-Barr virus DNA molecules in infectious mononucleosis-derived human lymphoid cell lines.
作者信息
Adams A, Bjursell G, Gussander E, Koliais S, Falk L, Lindahl T
出版信息
J Virol. 1979 Feb;29(2):815-7. doi: 10.1128/JVI.29.2.815-817.1979.
Abstract
The size of non-integrated circular Epstein-Barr virus (EBV) DNA molecules isolated from seven different human lymphoblastoid cell lines of infectious mononucleosis origin has been determined by sedimentation analysis and by direct contour length measurements on electron micrographs. Six lines had intracellular circular EBV genomes of the same size as linear virion DNA molecules. The seventh line, established with the B95-8 strain of EBV, was the only one found to have circular EBV DNA molecules significantly smaller than virion DNA. The data show that intracellular EBV DNA circles of reduced size do not generally occur in infectious mononucleosis-derived cell lines.
摘要
通过沉降分析以及对电子显微镜照片进行直接轮廓长度测量，已确定从7种源自传染性单核细胞增多症的不同人类淋巴母细胞系中分离出的非整合型环状爱泼斯坦-巴尔病毒（EBV）DNA分子的大小。6个细胞系具有与线性病毒粒子DNA分子大小相同的细胞内环状EBV基因组。用EBV的B95-8毒株建立的第7个细胞系是唯一发现具有明显小于病毒粒子DNA的环状EBV DNA分子的细胞系。数据表明，在源自传染性单核细胞增多症的细胞系中，一般不会出现大小减小的细胞内EBV DNA环。
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