在载体细胞中具有环状DNA分子特性的爱泼斯坦-巴尔病毒基因组。
Epstein-Barr virus genomes with properties of circular DNA molecules in carrier cells.
作者信息
Adams A, Lindahl T
出版信息
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1477-81. doi: 10.1073/pnas.72.4.1477.
A high-density fraction of high-molecular-weight DNA was isolated from the human lymphoid cell line Raji. This cell line contains 50 to 60 virus genome equivalents of Epstein-Barr virus DNA per cell, and the high-density DNA fraction was 10-fold enriched in such viral sequences. Sedimentation analysis on neutral glycerol gradients, followed by hybridization experiments with viral complementary RNA, showed that most of the intracellular viral DNA sequences in this material did not cosediment with the cellular DNA, but were recovered as two distinct species with sedimentation coefficients of 100 S and 65 S. These two forms sediment 1.70-1.75 and 1.10-1.12 times as fast as the linear Epstein-Barr virus DNA from virus particles, and thus have the hydrodynamic properties of a covalently closed circular form and a nicked (containing single-strand breaks) circular form of the virus genome. The 100S form also behaved as a covalently closed circular EBV DNA molecule on gradient centrifugation in CsC1/propidium diiodide, It would appear that latent Epstein-Barr virus DNA has the properties of a mammalian episome, and that both nonintegrated and integrated viral DNA sequences can be isolated from carrier cells.
从人淋巴母细胞系Raji中分离出高分子量DNA的高密度组分。该细胞系每个细胞含有50至60个埃-巴二氏病毒（EB病毒）DNA的病毒基因组当量，且高密度DNA组分中此类病毒序列富集了10倍。在中性甘油梯度上进行沉降分析，随后用病毒互补RNA进行杂交实验，结果表明该物质中大多数细胞内病毒DNA序列不与细胞DNA共同沉降，而是以沉降系数分别为100S和65S的两种不同形式回收。这两种形式的沉降速度分别是病毒颗粒中线性EB病毒DNA的1.70 - 1.75倍和1.10 - 1.12倍，因此具有病毒基因组共价闭合环状形式和带切口（含单链断裂）环状形式的流体动力学性质。在CsC1/碘化丙啶中进行梯度离心时，100S形式也表现为共价闭合环状EBV DNA分子。看来潜伏性EB病毒DNA具有哺乳动物附加体的性质，并且未整合和整合的病毒DNA序列都可以从携带细胞中分离出来。
Zotero 插件