爱泼斯坦-巴尔病毒转化的人淋巴瘤细胞系中的整合病毒DNA序列。
Integrated viral DNA sequences in Epstein-Barr virus-converted human lymphoma lines.
作者信息
Andersson-Anvret M, Lindahl T
出版信息
J Virol. 1978 Mar;25(3):710-8. doi: 10.1128/JVI.25.3.710-718.1978.
Abstract
Most human lymphoid cell lines contain multiple copies of circular, nonintegrated Epstein-Barr virus (EBV) DNA molecules as well as viral DNA sequences with properties of integrated DNA. The physical state of the EBV DNA in a human lymphoma line that only contains one virus genome equivalent per cell has now been studied by three different methods, neutral CsCl density gradient centrifugation, actinomycin D-CsCl gradient centrifugation, and Hirt fractionation. This cell line, AW-Ramos, has been obtained by EBV infection in vitro of the apparently EBV-negative Ramos lymphoma line. The results indicate that the EBV DNA in AW-Ramos is present exclusively in a linearly integrated form. Similar data were obtained with two other EBV-converted sublines of Ramos cells.
摘要
大多数人类淋巴细胞系含有多个环状、非整合的爱泼斯坦-巴尔病毒（EBV）DNA分子拷贝以及具有整合DNA特性的病毒DNA序列。现已通过三种不同方法研究了一种人淋巴瘤细胞系中EBV DNA的物理状态，该细胞系每个细胞仅含有一个病毒基因组当量，这三种方法分别是中性CsCl密度梯度离心、放线菌素D-CsCl梯度离心和Hirt分级分离。这个细胞系AW-Ramos是通过EBV体外感染明显EBV阴性的Ramos淋巴瘤细胞系获得的。结果表明，AW-Ramos中的EBV DNA仅以线性整合形式存在。用另外两个Ramos细胞的EBV转化亚系也获得了类似的数据。
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