Department of Behavioural Ecology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland.
Parasit Vectors. 2011 Sep 20;4:182. doi: 10.1186/1756-3305-4-182.
Anaplasmataceae and Borrelia burgdorferi s.l. are important tick-borne bacteria maintained in nature by transmission between ticks and vertebrate hosts. However, the potential role of lizards as hosts has not been sufficiently studied.
The current study showed that 23 of 171 examined sand lizards Lacerta agilis were PCR positive for Anaplasmataceae. The nucleotide sequences of the several selected PCR products showed 100% homology with Anaplasma spp. found in Ixodes ricinus collected in Tunisia and Morocco (AY672415 - AY672420). 1.2% of lizard collar scale samples were PCR positive for B. lusitaniae. In addition, 12 of 290 examined I. ricinus were PCR positive for B. burgdorferi s.l. and 82 were PCR positive for Anaplasmatacea. The number of ticks per lizard and the number of ticks PCR positive for both microorganisms per lizard were strongly correlated. Moreover, we found a significant correlation between numbers of ticks infected with Anaplasmataceae and with B. burgdorferi s.l. living on the same lizard. However, there was no significant correlation between detection of both bacteria in the same tick.
To the best of our knowledge, this is the first report of Anaplasmataceae DNA and additionally the second report of B. burgdorferi s.l DNA detection in the sand lizard.
阿纳姆体科和伯氏疏螺旋体属是通过蜱虫和脊椎动物宿主之间的传播在自然界中维持的重要蜱传细菌。然而，蜥蜴作为宿主的潜在作用尚未得到充分研究。
本研究显示，在 171 只被检查的沙蜥（Lacerta agilis）中，有 23 只 PCR 检测为阿纳姆体科阳性。从几种选定的 PCR 产物中获得的核苷酸序列与在突尼斯和摩洛哥采集的伊蚊（Ixodes ricinus）中发现的阿纳姆体属（AY672415-AY672420）完全同源。1.2%的蜥蜴领圈鳞片样本 PCR 检测为 B. lusitaniae 阳性。此外，在 290 只被检查的伊蚊中，有 12 只为 B. burgdorferi s.l. PCR 阳性，82 只为阿纳姆体科 PCR 阳性。每只蜥蜴上的蜱虫数量和每只蜥蜴上同时感染两种微生物的蜱虫数量呈强相关性。此外，我们发现感染阿纳姆体科的蜱虫数量与生活在同一蜥蜴上的伯氏疏螺旋体属的数量之间存在显著相关性。然而，在同一蜱虫中同时检测到两种细菌之间没有显著相关性。
据我们所知，这是首次在沙蜥中检测到阿纳姆体科 DNA，也是第二次检测到伯氏疏螺旋体属 DNA。
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