Zheng Gengfeng, Lieber Charles M
Laboratory of Advanced Materials, Department of Chemistry, Fudan University, Shanghai, China.
Methods Mol Biol. 2011;790:223-37. doi: 10.1007/978-1-61779-319-6_18.
Sensitive and quantitative analysis of proteins is central to disease diagnosis, drug screening, and proteomic studies. Among recent research advances exploiting new nanomaterials for biomolecule analysis, silicon nanowires (SiNWs), which are configured as field-effect transistors (FETs), have emerged as one of the most promising and powerful platforms for label-free, real-time, and highly sensitive electrical detection of proteins as well as many other biological species. Here, we describe a detailed protocol for realizing SiNW biosensors for protein detection that includes SiNW synthesis, FET device fabrication, surface receptor functionalization, and electrical sensing measurements. Moreover, incorporating both p-type and n-type SiNWs in the same sensor array provides a unique means of internal control for sensing signal verification.
蛋白质的灵敏定量分析是疾病诊断、药物筛选和蛋白质组学研究的核心。在利用新型纳米材料进行生物分子分析的最新研究进展中，被配置为场效应晶体管（FET）的硅纳米线（SiNW）已成为用于蛋白质以及许多其他生物物种的无标记、实时和高灵敏电学检测的最具潜力和强大的平台之一。在此，我们描述了一种用于实现蛋白质检测的SiNW生物传感器的详细方案，该方案包括SiNW合成、FET器件制造、表面受体功能化和电学传感测量。此外，在同一传感器阵列中同时纳入p型和n型SiNW为传感信号验证提供了一种独特的内部控制手段。
