在轨道阱质谱仪中实施光解。
Implementing photodissociation in an Orbitrap mass spectrometer.
机构信息
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA.
出版信息
J Am Soc Mass Spectrom. 2011 Jun;22(6):1105-8. doi: 10.1007/s13361-011-0119-7. Epub 2011 Apr 9.
Abstract
We modified a dual pressure linear ion trap Orbitrap to permit infrared multiphoton dissociation (IRMPD) in the higher energy collisional dissociation (HCD) cell for high resolution analysis. A number of parameters, including the pressures of the C-trap and HCD cell, the radio frequency (rf) amplitude applied to the C-trap, and the HCD DC offset, were evaluated to optimize IRMPD efficiency and maintain a high signal-to-noise ratio. IRMPD was utilized for characterization of phosphopeptides, supercharged peptides, and N-terminal modified peptides, as well as for top-down protein analysis. The high resolution and high mass accuracy capabilities of the Orbitrap analyzer facilitated confident assignment of product ions arising from IRMPD.
摘要
我们改进了双压线性离子阱轨道阱，使其能够在更高能量碰撞解离（HCD）池中进行红外多光子解离（IRMPD），以进行高分辨率分析。评估了许多参数，包括 C 阱和 HCD 池的压力、施加在 C 阱上的射频（rf）幅度以及 HCD 直流偏移，以优化 IRMPD 效率并保持高信噪比。IRMPD 用于磷酸肽、超荷质比肽和 N 端修饰肽的表征，以及用于从头蛋白质分析。轨道阱分析仪的高分辨率和高质量精度能力有助于对来自 IRMPD 的产物离子进行准确的归属。
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