Peng Ying, Bartley Laura E, Canlas Patrick, Ronald Pamela C
Department of Plant Pathology, University of California, Davis, CA 95616 USA.
Rice (N Y). 2010 Mar;3(1):36-42. doi: 10.1007/s12284-010-9039-6. Epub 2010 Feb 20.
WRKY transcription factors regulate diverse plant processes including responses to biotic stresses. Our previous studies indicate that OsWRKY62, an OsWRKY IIa subfamily member, functions as a negative regulator of the rice defense against Xanthomonas oryzae pv. oryzae. Here, we report that a large inverted repeat construct designed to knock down the expression of the four OsWRKY IIa subfamily members (OsWRKY62, OsWRKY28, OsWRKY71, and OsWRKY76) leads to overexpression of all four genes and disease resistance in some transgenic plants. These phenotypes are stably inherited as reflected by progeny analysis. A pathogenesis-related gene, PR10, is up-regulated in plants overexpressing the OsWRKY IIa genes. These results suggest that OsWRKY IIa proteins interact functionally to modulate plant innate immunity.
WRKY转录因子调控多种植物生理过程，包括对生物胁迫的响应。我们之前的研究表明，OsWRKY62作为OsWRKY IIa亚家族成员，在水稻抵御水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae）的过程中起负调控作用。在此，我们报道了一个设计用于敲低四个OsWRKY IIa亚家族成员（OsWRKY62、OsWRKY28、OsWRKY71和OsWRKY76）表达的大反向重复构建体，结果导致所有这四个基因在一些转基因植物中过表达并具有抗病性。如子代分析所示，这些表型能够稳定遗传。一个病程相关基因PR10在过表达OsWRKY IIa基因的植物中上调。这些结果表明，OsWRKY IIa蛋白在功能上相互作用以调节植物的先天免疫。
