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ONAC066，一种应激反应型NAC转录激活因子，通过调节病程相关蛋白基因和三个细胞色素P450基因的表达，对水稻抗稻瘟病免疫起正向作用。

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Through Modulating Expression of and Three Cytochrome P450 Genes.

作者信息

Yuan Xi, Wang Hui, Bi Yan, Yan Yuqing, Gao Yizhou, Xiong Xiaohui, Wang Jiajing, Li Dayong, Song Fengming

机构信息

National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, China.

出版信息

Front Plant Sci. 2021 Sep 9;12:749186. doi: 10.3389/fpls.2021.749186. eCollection 2021.

DOI:10.3389/fpls.2021.749186
PMID:34567053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8458891/
Abstract

NAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of in rice immunity. ONAC066 shows transcription activator activity that depends on its C-terminal region in rice cells. -OE plants exhibited enhanced resistance while -Ri and plants showed attenuated resistance to . A total of 81 genes were found to be up-regulated in -OE plants, and 26 of them were predicted to be induced by . Four genes, including and , were up-regulated in -OE plants but down-regulated in -Ri plants. ONAC066 bound to NAC core-binding site in promoter and activated expression, indicating that is a ONAC066 target. A set of cytochrome P450 genes were found to be co-expressed with and 5 of them were up-regulated in -OE plants but down-regulated in -Ri plants. ONAC066 bound to promoters of cytochrome P450 genes , , and and activated their transcription, indicating that these three cytochrome P450 genes are ONAC066 targets. These results suggest that ONAC066, as a transcription activator, positively contributes to rice immunity through modulating the expression of and a set of cytochrome P450 genes to activate defense response.

摘要

NAC转录因子在水稻中构成一个大家族，其中一些已被证明在水稻免疫中发挥关键作用。本研究调查了[具体基因名称未给出]在水稻免疫中的功能和机制。ONAC066在水稻细胞中表现出依赖其C端区域的转录激活活性。[过表达基因名称未给出]-OE植株表现出增强的抗性，而[RNA干扰基因名称未给出]-Ri和[基因敲除基因名称未给出]植株对[病原体名称未给出]的抗性减弱。共发现81个基因在[过表达基因名称未给出]-OE植株中上调，其中26个预计由[病原体名称未给出]诱导。包括[具体基因名称未给出]和[具体基因名称未给出]在内的4个[具体基因名称未给出]基因在[过表达基因名称未给出]-OE植株中上调，但在[RNA干扰基因名称未给出]-Ri植株中下调。ONAC066与[具体基因名称未给出]启动子中的NAC核心结合位点结合并激活[具体基因名称未给出]表达，表明[具体基因名称未给出]是ONAC066的靶标。发现一组细胞色素P450基因与[具体基因名称未给出]共表达，其中5个在[过表达基因名称未给出]-OE植株中上调，但在[RNA干扰基因名称未给出]-Ri植株中下调。ONAC066与细胞色素P450基因[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]的启动子结合并激活它们的转录，表明这三个细胞色素P450基因是ONAC066的靶标。这些结果表明，ONAC066作为一种转录激活剂，通过调节[具体基因名称未给出]和一组细胞色素P450基因的表达来激活防御反应，从而对水稻免疫产生积极贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/c44488493fa5/fpls-12-749186-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/f41b416b6cf6/fpls-12-749186-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/827b05c41b66/fpls-12-749186-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/4579cf91c3b3/fpls-12-749186-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/39f9363a9c1e/fpls-12-749186-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/674c41013cf5/fpls-12-749186-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/c44488493fa5/fpls-12-749186-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/f41b416b6cf6/fpls-12-749186-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/827b05c41b66/fpls-12-749186-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/4579cf91c3b3/fpls-12-749186-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/39f9363a9c1e/fpls-12-749186-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/674c41013cf5/fpls-12-749186-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b79c/8458891/c44488493fa5/fpls-12-749186-g006.jpg

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