Virus Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Virology. 2012 Jan 5;422(1):1-5. doi: 10.1016/j.virol.2011.08.024. Epub 2011 Oct 2.
In October and November 2010, hospitals in northern Uganda reported patients with suspected hemorrhagic fevers. Initial tests for Ebola viruses, Marburg virus, Rift Valley fever virus, and Crimean Congo hemorrhagic fever virus were negative. Unbiased PCR amplification of total RNA extracted directly from patient sera and next generation sequencing resulted in detection of yellow fever virus and generation of 98% of the virus genome sequence. This finding demonstrated the utility of next generation sequencing and a metagenomic approach to identify an etiological agent and direct the response to a disease outbreak.
2010 年 10 月和 11 月，乌干达北部的医院报告了疑似出血热患者。对埃博拉病毒、马尔堡病毒、裂谷热病毒和克里米亚-刚果出血热病毒的初始检测结果为阴性。直接从患者血清中提取的总 RNA 的无偏 PCR 扩增和下一代测序导致检测到黄热病病毒，并生成病毒基因组序列的 98%。这一发现证明了下一代测序和宏基因组方法在确定病因和指导疾病暴发应对方面的实用性。
