Tang Xinhui, Yan Lifang, Gao Jing, Ge Haiyan, Yang Haidong, Lin Na
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002.
Pharmacogn Mag. 2011 Jul;7(27):186-92. doi: 10.4103/0973-1296.84225.
To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) MATERIALS AND METHODS: One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro.
According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity.
One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.
优化白花丹科植物中华补血草根部多糖的提取工艺，并评价中华补血草多糖（LSEP）的抗氧化能力。材料与方法：采用单因素法和响应面法（RSM）从中华补血草中提取多糖。然后，建立1,1-二苯基-2-苦基肼自由基、羟基自由基（·OH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐自由基测定法，以测定LSEP的体外抗氧化能力。
分析表明，提取温度对提取率有显著影响。LSEP的最佳提取条件如下：提取温度95℃；超声时间50分钟；料液比1:12。在此条件下，粗LSEP的实验产率为12.80±0.19%，与预测模型吻合良好。抗氧化能力数据表明LSEP具有较强的抗氧化活性。
采用单因素法和响应面法提取LSEP简单可行，且LSEP因其较强的抗氧化活性可开发为营养保健品。
