Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University Belfast, Northern Ireland, UK.
Toxicon. 2011 Dec 1;58(8):619-22. doi: 10.1016/j.toxicon.2011.09.012. Epub 2011 Sep 29.
A flagellar protein belonging to the genus Methylobacterium or Agrobacterium was previously observed by proteomics in azaspiracids (AZA) toxic mussels. Here, we report the isolation of two different Methylobacterium spp. (NTx1 and Tx1) from non-toxic and AZA toxic mussels, respectively, which when co-cultured with AZA exhibited significantly different growth responses - isolate Tx1 growth rate was enhanced, whereas growth of isolate NTx1 was adversely affected, compared to non-AZA supplemented control cultures. A Hyphomicrobium sp. (Tx2) also isolated from the toxic mussels achieved greater cell density in AZAs supplemented cultures.
先前通过蛋白质组学在含有麻痹性贝类毒素的笠贝中观察到一种属于甲基杆菌属或土壤杆菌属的鞭毛蛋白。在这里，我们分别从无毒和含有麻痹性贝类毒素的笠贝中分离出两种不同的甲基杆菌属（NTx1 和 Tx1），当与 AZA 共同培养时，它们表现出明显不同的生长反应 - 与不含 AZA 的对照培养物相比，分离株 Tx1 的生长速率得到增强，而分离株 NTx1 的生长则受到不利影响。从有毒笠贝中分离出的一株食酸菌属（Tx2）在添加 AZA 的培养物中也达到了更高的细胞密度。
