Obembe Agona O, Owu Daniel U, Okwari Obem O, Antai Atim B, Osim Eme E
Department of Physiology, College of Medical Sciences, University of Calabar, 540001 Calabar, Nigeria.
ISRN Gastroenterol. 2011;2011:972838. doi: 10.5402/2011/972838. Epub 2010 Oct 26.
Chronic ingestion of thermoxidized palm oil causes functional derangement of various tissues. This study was therefore carried out to determine the effect of chronic ingestion of thermoxidized and fresh palm oil diets on intestinal fluid and glucose absorption in rats using the everted sac technique. Thirty Wistar rats were divided into three groups of 10 rats per group. The first group was the control and was fed on normal rat chow while the second (FPO) and third groups (TPO) were fed diet containing either fresh or thermoxidized palm oil (15% wt/wt) for 14 weeks. Villus height and crypt depth were measured. The gut fluid uptake and gut glucose uptake were significantly (P < .001) lower in the TPO group than those in the FPO and control groups, respectively. The villus height in the TPO was significantly (P < .01) lower than that in FPO and control. The villus depth in TPO was significantly (P < .05) higher than that in FPO and control groups, respectively. These results suggest that ingestion of thermoxidized palm oil and not fresh palm oil may lead to distortion in villus morphology with a concomitant malabsorption of fluid and glucose in rats due to its harmful free radicals.
长期摄入热氧化棕榈油会导致各种组织的功能紊乱。因此，本研究采用外翻肠囊技术，旨在确定长期摄入热氧化棕榈油和新鲜棕榈油饮食对大鼠肠液和葡萄糖吸收的影响。30只Wistar大鼠被分为三组，每组10只。第一组为对照组，喂食正常大鼠饲料，而第二组（FPO）和第三组（TPO）则喂食含有新鲜或热氧化棕榈油（15%重量/重量）的饲料，持续14周。测量绒毛高度和隐窝深度。TPO组的肠液摄取和肠葡萄糖摄取分别显著低于FPO组和对照组（P < .001）。TPO组的绒毛高度显著低于FPO组和对照组（P < .01）。TPO组的绒毛深度分别显著高于FPO组和对照组（P < .05）。这些结果表明，摄入热氧化棕榈油而非新鲜棕榈油可能会导致大鼠绒毛形态畸变，并因其有害自由基而伴随液体和葡萄糖吸收不良。
