ICMR virus unit, GB-4, 1st Floor, ID & BG Hospital, 57 Dr. S. C. Banerjee Road, Beliaghata, Kolkata-700010, India.
Trans R Soc Trop Med Hyg. 2012 Jan;106(1):15-9. doi: 10.1016/j.trstmh.2011.08.011. Epub 2011 Oct 11.
Japanese encephalitis (JE), a neurotrophic disease, was first recorded in the state of West Bengal, India in 1973. Since then JE is being reported every year from different districts. With a view to identify the JE cases accurately, a study was undertaken to detect the Japanese encephalitis virus (JEV) as the etiologic agent from the acute encephalitis syndrome (AES) cases and to identify its distribution in different districts. We report the results of 513 blood or cerebrospinal fluid samples referred/collected from the hospitalized AES cases. The samples were initially subjected to Mac-ELISA test followed by reverse transcriptase (RT)-PCR for the detection of IgM antibodies and the JEV genome, specific to E gene, respectively. Out of 513 samples referred/collected, 139 (27.1%) samples were reactive to JE IgM antibody. The remaining 374 samples were screened to select those which had a history of illness with a duration of ≤3 days. Only 147 samples were selected and tested, out of which 36 (24.5%) isolates were achieved and those were RT-PCR positive against the control JEV strain. Detection of IgM antibody to JE and the RT-PCR result confirms the active circulation of JEV in different districts of West Bengal and needs to be monitored carefully.
日本脑炎（JE）是一种神经毒病，于 1973 年在印度西孟加拉邦首次记录。此后，每年都有不同地区报告 JE 病例。为了准确识别 JE 病例，进行了一项研究，以检测日本脑炎病毒（JEV）作为病因，并确定其在不同地区的分布。我们报告了从住院 AES 病例中送检/收集的 513 份血液或脑脊液样本的结果。这些样本首先进行 Mac-ELISA 检测，然后进行逆转录（RT）-PCR，以分别检测针对 E 基因的 IgM 抗体和 JEV 基因组。在送检/收集的 513 份样本中，有 139 份（27.1%）样本对 JE IgM 抗体呈反应性。其余 374 个样本被筛选以选择那些有≤3 天病程的病史。仅选择和测试了 147 个样本，其中 36 个（24.5%）分离株获得了阳性结果，这些分离株对对照 JEV 株的 RT-PCR 呈阳性。JE 的 IgM 抗体检测和 RT-PCR 结果证实了 JEV 在西孟加拉邦不同地区的活跃传播，需要仔细监测。
