Clancy A N, Bonsall R W, Michael R P
Department of Psychiatry, Emory University School of Medicine, Atlanta, GA 30322.
Life Sci. 1992;50(6):409-17. doi: 10.1016/0024-3205(92)90375-y.
Androgen receptor antibodies have recently been developed using fusion proteins containing fragments of human prostatic androgen receptor. We have used a polyclonal antibody raised in rabbits to label androgen receptors in brain sections from male and female rats and monkeys. Free-floating frozen sections were incubated in primary antibody, and processed by the peroxidase-avidin-biotin complex method using biotinylated anti-rabbit IgG. Nickel intensified diaminobenzidine was used as the chromagen, and neurons were labeled in the amygdala, hippocampus, bed nucleus of stria terminalis, septum, preoptic area, in several hypothalamic nuclei including the supraoptic and paraventricular nuclei, in several brain stem motor nuclei and in cerebral cortex. Staining was most intense in cell nuclei but also occurred in cytoplasm and in some neuronal processes. Labeling was more restricted in monkey than in rat brain. Omitting the primary antibody or pre-incubating the primary antibody with rat prostatic cytosol for control purposes demonstrated the specificity of staining.
最近，利用包含人前列腺雄激素受体片段的融合蛋白开发出了雄激素受体抗体。我们使用在兔体内产生的多克隆抗体对雄性和雌性大鼠及猴子脑切片中的雄激素受体进行标记。将游离漂浮的冷冻切片在一抗中孵育，然后使用生物素化抗兔IgG通过过氧化物酶-抗生物素蛋白-生物素复合物法进行处理。使用镍增强的二氨基联苯胺作为显色剂，在杏仁核、海马体、终纹床核、隔区、视前区、包括视上核和室旁核在内的几个下丘脑核、几个脑干运动核以及大脑皮层中标记出神经元。染色在细胞核中最为强烈，但也出现在细胞质和一些神经突起中。与大鼠脑相比，猴子脑中的标记更为局限。省略一抗或为了对照目的将一抗与大鼠前列腺胞质溶胶预孵育，证明了染色的特异性。
