Department of Clinical Microbiology, Odense University Hospital, Winsløwparken 21, 2, DK-5000 Odense C, Denmark.
J Clin Microbiol. 2011 Dec;49(12):4314-8. doi: 10.1128/JCM.05788-11. Epub 2011 Oct 12.
We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.
我们比较了两种基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统（岛津/SARAMIS 和布鲁克）对连续收集的临床重要厌氧细菌（n = 290）的检测结果。布鲁克系统在种水平上的正确鉴定率更高（67.2%对 49.0%），但错误鉴定率也更高（7.9%对 1.4%）。系统数据库需要进行优化以提高鉴定水平。然而，MALDI-TOF MS 在目前的版本中似乎是一种快速且廉价的方法，可用于鉴定大多数临床重要的厌氧细菌。
