Hubbs-SeaWorld Research Institute, Center for Marine Veterinary Virology, 2595 Ingraham St., San Diego, CA 92109, USA.
Vet Microbiol. 2012 Mar 23;155(2-4):257-66. doi: 10.1016/j.vetmic.2011.09.027. Epub 2011 Oct 1.
A seven-year old California sea lion (Zalophus californianus) presented with focally extensive, bilaterally symmetric, proliferative axillary skin lesions and preputial lesions. A second California sea lion in the same population presented with similar proliferative lesions on the underside of the tail. Histopathology revealed epidermal hyperplasia with severe hyperkeratosis, with proliferating keratinocytes forming broad, branching pegs that extended into the dermis. Pan-papillomaviral consensus PCR was used to obtain initial E1 sequence template and the complete genome was determined using a combination of rolling circle amplification and specific-primer PCR. Analysis revealed a novel papillomavirus, Zalophus californianus papillomavirus 1 (ZcPV1), with seven open reading frames encoding five early proteins (E6, E7, E1, E2 and E4) and two late proteins (L1 and L2). Phylogenetic analysis revealed that (ZcPV1) is most closely related to Equine papillomavirus 1 (EcPV1) in the genus Zetapapillomavirus, and Canine papillomaviruses 3 and 4 (CPV3, CPV4) in the genus Chipapillomavirus. The lesions regressed without intervention over a period of several months.
一只七岁的加利福尼亚海狮（Zalophus californianus）表现出局限性广泛、双侧对称、增生性腋部皮肤病变和包皮病变。同一群体中的另一只加利福尼亚海狮的尾巴下方也出现了类似的增生性病变。组织病理学显示表皮增生伴严重角化过度，增生的角质形成细胞形成宽阔的分支钉突，延伸至真皮。使用泛乳头瘤病毒共识 PCR 获得初始 E1 序列模板，并使用滚环扩增和特异性引物 PCR 的组合确定完整基因组。分析显示了一种新型的乳头瘤病毒，加利福尼亚海狮乳头瘤病毒 1（ZcPV1），具有七个开放阅读框，编码五个早期蛋白（E6、E7、E1、E2 和 E4）和两个晚期蛋白（L1 和 L2）。系统发育分析表明，（ZcPV1）与 Zetapapillomavirus 属的马乳头瘤病毒 1（EcPV1）以及 Chipapillomavirus 属的犬乳头瘤病毒 3 和 4（CPV3、CPV4）最为密切相关。病变在几个月的时间内未经干预而消退。
