Department of Oral and Maxillofacial Diagnostic Sciences, College of Dentistry, University of Florida, Gainesville, FL 32610, USA.
Int J Oral Sci. 2011 Oct;3(4):209-15. doi: 10.4248/IJOS11075.
Periodontal disease has been recently linked to a variety of systemic conditions such as diabetes, cardiovascular disease, preterm delivery, and oral cancer. The most common bacteria associated with periodontal disease, Porphyromonas gingivalis (P. gingivalis) has not yet been studied in the malignant gingival tissues. The objective of this study was to investigate the presence of P. gingivalis in specimens from squamous cell carcinoma patients. We have performed immunohistochemical staining to investigate the presence of P. gingivalis and Streptococcus gordonii (S. gordonii), a non invasive oral bacteria, in paraffin embedded samples of gingival squamous cell carcinoma (n = 10) and normal gingiva (n = 5). Staining for P. gingivalis revealed the presence of the bacteria in normal gingival tissues and gingival carcinoma, with higher levels (more than 33%, P < 0.05) detected in the carcinoma samples. The staining intensity was also significantly enhanced in the malignant tissue by 2 folds (P < 0.023) compared to specimens stained for the non-invasive S. gordonii. P. gingivalis is abundantly present in malignant oral epithelium suggesting a potential association of the bacteria with gingival squamous cell carcinoma.
牙周病最近与多种系统性疾病相关，如糖尿病、心血管疾病、早产和口腔癌。与牙周病最相关的常见细菌牙龈卟啉单胞菌（P. gingivalis）尚未在恶性牙龈组织中进行研究。本研究的目的是调查牙龈鳞状细胞癌患者标本中是否存在 P. gingivalis。我们进行了免疫组织化学染色，以研究牙龈鳞状细胞癌（n=10）和正常牙龈（n=5）石蜡包埋样本中牙龈卟啉单胞菌和非侵入性口腔细菌戈登链球菌（S. gordonii）的存在情况。牙龈卟啉单胞菌染色显示该细菌存在于正常牙龈组织和牙龈癌中，癌组织中的细菌含量更高（超过 33%，P<0.05）。与非侵入性 S. gordonii 染色的标本相比，恶性组织中的染色强度也显著增强了 2 倍（P<0.023）。牙龈卟啉单胞菌大量存在于恶性口腔上皮组织中，表明其与牙龈鳞状细胞癌存在潜在关联。
