Joveen-Neoh Wan Fen, Chong Ka Lung, Wong Clemente Michael Vui Ling, Lau Tiek Ying
Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
J Parasitol Res. 2011;2011:104284. doi: 10.1155/2011/104284. Epub 2011 Oct 13.
Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region.
引言。疟疾是目前由疟原虫属寄生虫引起的最普遍的寄生虫传播疾病之一。基于显微镜检查对人类疟原虫尤其是诺氏疟原虫的错误鉴定非常常见。本文的目的是基于小亚基核糖体RNA（ssrRNA）准确鉴定马来西亚婆罗洲沙巴内陆地区人类疟原虫的发病率，并确定人类疟原虫的错误鉴定率。方法。采用巢式PCR检测人类疟原虫的存在。本研究共使用了243份来自要求进行疟原虫血片分析（BFMP）的患者的血斑样本。结果。巢式PCR结果显示没有间日疟原虫感染，最常见的疟原虫是诺氏疟原虫，其次是间日疟原虫、恶性疟原虫和混合感染。243份样本中只有69.5%的巢式PCR结果与显微镜检查结果一致。结论。疟疾分子检测的初步结果表明，诺氏疟原虫是沙巴内陆地区最常见的疟原虫种类。本文的研究结果可能为该地区不同疟原虫种类的实际传播情况提供更清晰的图景。
