南非药用植物的乙酰胆碱酯酶抑制和抗氧化活性的体外筛选。

In vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from southern Africa.

机构信息

Department of Pharmacology, School of Medicine, University of Pretoria, South Africa.

出版信息

Asian Pac J Trop Med. 2011 Oct;4(10):829-35. doi: 10.1016/S1995-7645(11)60203-4.

DOI:10.1016/S1995-7645(11)60203-4

PMID:22014742

Abstract

OBJECTIVE

To determine the acetylcholinesterase inhibitory (AChEI) and antioxidant activity of the ethyl acetate and methanol extracts of 12 traditional medicinal plants used in the treatment of neurological disorders.

METHODS

AChEI activity was determined spectrophotometrically using the Ellman's colorimetric method. Antioxidant activity was carried out by determining the ability of the extracts to scavenge 2,2-diphenyl-1-picryl hydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals. The levels of total phenols, flavonoids and flavonols were determined quantitatively using spectrophotometric methods.

RESULTS

AChEI was observed to be dose-dependent. Lannea schweinfurthii (L. schweinfurthii) (Engl.) Engl. and Scadoxus puniceus (S. puniceus) (L.) Friis & I. Nordal. root extracts showed the lowest IC(50) value of 0.000 3 mg/mL for the ethyl acetate extracts while Zanthoxylum davyi (Z. davyi) (I. Verd.) P.G. Watermann had the lowest IC(50) value of 0.01 mg/mL for the methanol extracts in the AChEI assay. The roots of Piper capense (P. capense) L.f., L. schweinfurthii, Ziziphus mucronata (Z. mucronata) Willd., Z. davyi and Crinum bulbispermum (C. bulbispermum) (Burm.f.) Milne-Redh. & Schweick. showed noteworthy radical scavenging activity and good AChEI activity.

CONCLUSIONS

Five plants show good antioxidant and AChEI activity. These findings support the traditional use of the plants for treating neurological disorders especially where a cholinesterase mechanism and reactive oxygen species (ROS) are involved.

摘要

目的

测定 12 种传统药用植物的乙酸胆碱酯酶抑制（AChEI）和抗氧化活性，这些植物用于治疗神经紊乱。

方法

使用 Ellman 的比色法分光光度法测定 AChEI 活性。通过测定提取物清除 2,2-二苯基-1-苦基肼（DPPH）和 2,2'-联氮双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基的能力来进行抗氧化活性。使用分光光度法定量测定总酚、类黄酮和黄酮醇的含量。

结果

AChEI 呈剂量依赖性。Lannea schweinfurthii（L. schweinfurthii）（Engl.）Engl.和 Scadoxus puniceus（S. puniceus）（L.）Friis & I. Nordal.根提取物的乙酸乙酯提取物对乙酰胆碱酯酶的 IC50 值最低，为 0.0003mg/mL，甲醇提取物的 IC50 值最低，为 0.01mg/mL，在 AChEI 测定中为 Zanthoxylum davyi（Z. davyi）（I. Verd.）P.G. Watermann。Piper capense（P. capense）L.f.、L. schweinfurthii、Ziziphus mucronata（Z. mucronata）Willd.、Z. davyi 和 Crinum bulbispermum（C. bulbispermum）（Burm.f.）Milne-Redh. & Schweick.的根表现出显著的自由基清除活性和良好的 AChEI 活性。