Nishiwaki H, Satake K, Umeyama K
First Department of Surgery, Osaka City University Medical School, Japan.
Jpn J Surg. 1990 Jul;20(4):418-23. doi: 10.1007/BF02470825.
Various cannulae have been devised to serve in experiments for collecting pancreatic juice. In this study we created a pancreatic fistula in dogs through a modification of Herrera's method. Since it is advisable to make as small a duodenal pouch as possible to collect almost all the secreted pancreatic juice, both ends of the pouch were closed by an inverted continuous all-layer suture. The lateral flange of the cannula was then introduced into the pouch to sample the pancreatic juice. Duodenoduodenostomy was performed to restore continuity of the alimentary tract, and the other end was inserted into the duodenum 3 cm distal to the anastomosis on the anal side. The exocrine pancreatic secretion of these dogs responded well to food ingestion, with a peak level of 14.5 +/- 5.4 ml/15 min appearing after 30 to 45 min in a postprandial state. Moreover, the animals were able to survive and be utilized for experiments for a period of 3 to 5 months. This experimental model is therefore considered to be of great value for the investigation of exocrine pancreatic secretion.
人们设计了各种插管用于收集胰液的实验。在本研究中，我们通过改进埃雷拉的方法在狗身上制造了胰瘘。由于制作尽可能小的十二指肠袋以收集几乎所有分泌的胰液是可取的，所以该袋的两端用连续全层内翻缝合封闭。然后将插管的侧凸缘插入袋中以采集胰液样本。进行十二指肠十二指肠吻合术以恢复消化道的连续性，另一端插入距吻合口肛门侧3厘米处的十二指肠。这些狗的胰腺外分泌对食物摄入反应良好，餐后30至45分钟时峰值水平为14.5±5.4毫升/15分钟。此外，这些动物能够存活并用于实验3至5个月。因此，该实验模型被认为对胰腺外分泌的研究具有重要价值。
