Department of Parasitology, University of Agriculture, Faisalabad-38040-Pakistan.
Parasitology. 2012 Feb;139(2):237-43. doi: 10.1017/S003118201100182X. Epub 2011 Oct 24.
The objective of the present study was to evaluate the anticoccidial effect of different concentrations of the herbal complex of 4 plants (leaves of Azadirachta indica and Nicotiana tabacum, flowers of Calotropis procera and seeds of Trachyspermum ammi) in broiler chickens in comparison with amprolium anticoccidial. Three concentrations (2 g, 4 g and 6 g) of herbal complex were given to the experimental groups once a day and amprolium (at the dose rate of 125 ppm) was given orally in drinking water from the 14th to the 21st days of age. One group was kept as infected, non-medicated control and one as non-infected, non-medicated control. All groups were inoculated orally with 75,000 sporulated oocysts on the 14th day of age except the non-infected, non-medicated control. Among herbal complex medicated groups, the maximum anticoccidial effect was seen in the group medicated with 6 g herbal complex followed by 4 g and 2 g herbal complex medicated groups. Treatment with 6 g of the herbal complex significantly reduced the negative performance and pathogenic effects associated with Eimeria tenella challenge at a level that was comparable with amprolium when using a largely susceptible recent field isolate. In summary, concentration-dependent anticoccidial activity of the studied herbal complex suggests its use as an alternative anticoccidial agent to chemotherapeutic drugs for Eimeria tenella control.
本研究的目的是评估 4 种植物（印楝叶和烟草叶、牛角瓜花和枯茗子）草药复合物不同浓度对肉鸡的抗球虫效果，并与氨丙啉抗球虫药进行比较。实验组每天口服一次 3 种不同浓度（2 g、4 g 和 6 g）的草药复合物，14 至 21 日龄时在饮水中口服氨丙啉（剂量率为 125 ppm）。一组作为感染、未用药对照，一组作为未感染、未用药对照。除未感染、未用药对照外，所有组均于 14 日龄时经口接种 75,000 个孢子化卵囊。在接受草药复合物治疗的组中，以 6 g 草药复合物治疗的组的抗球虫效果最佳，其次是 4 g 和 2 g 草药复合物治疗的组。使用一种高度敏感的近期田间分离株时，6 g 草药复合物的治疗可显著降低柔嫩艾美耳球虫攻毒引起的负面表现和病理效应，其效果可与氨丙啉相媲美。总之，所研究的草药复合物具有浓度依赖性抗球虫活性，表明其可作为化学治疗药物的替代品，用于柔嫩艾美耳球虫的控制。
