Fatemi Ahmadreza, Asasi Keramat, Razavi Seyed Mostafa
Department of Clinical Sciences, Veterinary School, Shiraz University, P.O. Box 1731, Shiraz, 71345, Iran.
Department of Pathobiology, Veterinary School, Shiraz University, Shiraz, Iran.
Parasitol Res. 2017 Sep;116(9):2581-2589. doi: 10.1007/s00436-017-5567-y. Epub 2017 Aug 1.
The effect of Artemisia annua ethanolic extract (AE) as a potential source of herbal anticoccidial activity was investigated on experimental coccidiosis in chicken. One hundred ninety-two one-day-old chicks were divided in to 8 groups (n = 24) including AE prevention group, AE-treated group, simultaneously challenged AE-medicated group, challenged-untreated group (positive control), unchallenged-untreated group (negative control), salinomycine prevention group, salinomycine-treated group, and simultaneously challenged salinomycine-medicated group, in a completely randomized design. Oral challenge carried out by a suspension containing a mixture of 200,000 oocysts Eimeria acervulina, 30,000 oocysts Eimeria necatrix, and 20,000 oocysts Eimeria tenella on day 21 of age. Weight gain in AE prevention group significantly increased compared to positive control group (p < 0.05). Unlike salinomycine prevention group, the food conversion ratio (FCR) of AE prevention group was not significantly higher than negative control. Oocyst per gram (OPG) in simultaneously challenged AE-medicated group had no significant difference, while for 38% of the days, in simultaneously challenged salinomycine-medicated group significantly decreased (p < 0.05). The food intake of AE-treated group had no significant difference with salinomycine-treated group (p > 0.05). In half of the days of OPGs sampling, AE-treated group was reduced significantly compared to positive control group (p < 0.05). Collectively, the in vivo study of anticoccidial effects of AE in the prevention section was more effective than the treatment section, while the treatment section was more effective than the simultaneous section. We concluded that AE has a potential value to use as an herbal medicine for preventive measure in chicken coccidiosis.
研究了青蒿乙醇提取物（AE）作为一种潜在的草药抗球虫活性来源对鸡实验性球虫病的影响。192只1日龄雏鸡被分为8组（每组n = 24），包括AE预防组、AE治疗组、同时攻毒的AE给药组、攻毒未治疗组（阳性对照）、未攻毒未治疗组（阴性对照）、盐霉素预防组、盐霉素治疗组和同时攻毒的盐霉素给药组，采用完全随机设计。在第21日龄时，通过含有200,000个堆型艾美耳球虫卵囊、30,000个毒害艾美耳球虫卵囊和20,000个柔嫩艾美耳球虫卵囊混合物的悬浮液进行口服攻毒。与阳性对照组相比，AE预防组的体重增加显著（p < 0.05）。与盐霉素预防组不同，AE预防组的饲料转化率（FCR）不显著高于阴性对照。同时攻毒的AE给药组每克粪便中的卵囊数（OPG）无显著差异，而在同时攻毒的盐霉素给药组中，有38%的天数显著减少（p < 0.05）。AE治疗组的采食量与盐霉素治疗组无显著差异（p > 0.05）。在OPG采样的一半天数中，AE治疗组与阳性对照组相比显著降低（p < 0.05）。总体而言，AE抗球虫作用的体内研究在预防组比治疗组更有效，而治疗组比同时给药组更有效。我们得出结论，AE作为预防鸡球虫病的草药具有潜在价值。
