Lowndes N F, Bushel P, Mendelsohn L, Wu J, Yen M Y, Allan M
Department of Genetics, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Mol Cell Biol. 1990 Sep;10(9):4990-5. doi: 10.1128/mcb.10.9.4990-4995.1990.
We have identified a short, highly repetitive element within intron -1 of the human c-Ha-ras gene. This element was found to be transcribed in both orientations and to be homologous to heterogeneous nonpolyadenylated transcripts. The repetitive element blocked transcriptional readthrough from a strong upstream viral promoter but allowed unimpaired readthrough from the c-Has-ras promoter. We suggest that it may serve to prevent excessive transcription into the coding region of the gene under such circumstances as viral insertion.
我们在人类c-Ha-ras基因的内含子-1中鉴定出一个短的、高度重复的元件。发现该元件以两种方向转录，并且与异质非聚腺苷酸化转录本同源。该重复元件阻断了来自强上游病毒启动子的转录通读，但允许来自c-Has-ras启动子的通读不受影响。我们认为，在病毒插入等情况下，它可能有助于防止过度转录进入该基因的编码区。
