Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA.
Nat Struct Mol Biol. 2011 Oct 23;18(11):1300-2. doi: 10.1038/nsmb.2140.
During protein synthesis, mRNA and tRNAs are iteratively translocated by the ribosome. Precisely what molecular event is rate limiting for translocation is not known. Here we show that disruption of the interactions between the A-site codon and the ribosome accelerates translocation, suggesting that the release of the mRNA from the decoding center of the ribosome is the rate-limiting step of translocation. These results provide insight into the molecular mechanism of translocation.
在蛋白质合成过程中，mRNA 和 tRNA 被核糖体反复转移。目前尚不清楚核糖体转移的限速分子事件是什么。在这里，我们表明，破坏 A 位密码子与核糖体之间的相互作用会加速转移，这表明 mRNA 从核糖体的解码中心释放是核糖体转移的限速步骤。这些结果为核糖体转移的分子机制提供了新的见解。
