Smeenk R, Brinkman K, van den Brink H, Swaak T
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service.
Clin Rheumatol. 1990 Mar;9(1 Suppl 1):63-72. doi: 10.1007/BF02205553.
In this paper we will briefly compare four assays in use in our institute for the measurement of antibodies to DNA: the anti-DNA ELISA, the PEG assay, the indirect immunofluorescence test on Crithidia luciliae and the Farr assay. Although with the use of sera from defined patients with systemic lupus erythematosus (SLE) quite good correlations were obtained between the various assays, these correlations were lost upon the use in routine screening of sera of undefined patients. It will be shown that the Farr assay has the highest specificity to systemic lupus erythematosus, whereas the ELISA and the PEG assay are the most sensitive methods. In its present form, however, the ELISA is not suited for the detection of IgM anti-DNA. Furthermore, this technique alone also detects DNA/-anti-DNA complexes present in sera or hybridoma culture supernatants.
在本文中，我们将简要比较我院用于检测抗DNA抗体的四种检测方法：抗DNA酶联免疫吸附测定（ELISA）、聚乙二醇（PEG）测定、对利什曼原虫的间接免疫荧光试验以及法尔（Farr）测定。尽管使用来自明确诊断的系统性红斑狼疮（SLE）患者的血清时，各种检测方法之间获得了相当好的相关性，但在对未明确诊断患者的血清进行常规筛查时，这些相关性就消失了。结果将表明，法尔测定对系统性红斑狼疮具有最高的特异性，而ELISA和PEG测定是最敏感的方法。然而，以其目前的形式，ELISA不适合检测IgM抗DNA。此外，仅这项技术也能检测血清或杂交瘤培养上清液中存在的DNA/抗DNA复合物。
