Mayo Clinic College of Medicine, Department of Orthopedic Surgery, Rochester, MN, USA.
J Biol Regul Homeost Agents. 2011 Apr-Jun;25(2 Suppl):S15-23.
Polypropylene fumarate (PPF) scaffolds fabricated by rapid prototyping were surface modified by solution deposition of electrically conductive polypyrrole coatings with or without hydroxyapatite. Scaffolds were electrically conductive with resistivity as low as 2Ω. Scaffold characterization by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis shows both polypyrrole and hydroxyapatite are present. Cell viability, attachment, proliferation, and differentiation were analyzed using human fetal osteoblast cells. These studies show that surface modification using hydroxyapatite improved cell attachment and proliferation of osteoblasts onto the PPF scaffolds. Alkaline phosphatase activity as a marker for osteogenic differentiation of cell to mature osteoblasts was analyzed. Our data reveal that osteoblasts maintained their phenotype on PPF scaffolds with and without coatings. Thus, these scaffolds could be appropriate candidates for our future in vivo studies.
聚反丁烯二酸酯（PPF）支架通过快速原型制造，通过电导率聚吡咯涂层的溶液沉积进行表面改性，有或没有羟基磷灰石。支架具有低至 2Ω 的电阻率，通过傅里叶变换红外光谱、X 射线光电子能谱和热重分析对支架进行了表征，结果表明聚吡咯和羟基磷灰石都存在。使用人胎成骨细胞分析细胞活力、附着、增殖和分化。这些研究表明，使用羟基磷灰石进行表面改性可以提高成骨细胞在 PPF 支架上的附着和增殖。碱性磷酸酶活性作为细胞向成熟成骨细胞的成骨分化的标志物进行分析。我们的数据表明，成骨细胞在有涂层和无涂层的 PPF 支架上都能保持其表型。因此，这些支架可能是我们未来体内研究的合适候选物。
