日粮高蛋白通过激活仔猪的NF-κB信号通路诱导腹泻和肠道炎症。
Dietary high protein-induced diarrhea and intestinal inflammation by activation of NF-κB signaling in piglets.
作者信息
Yin Lanmei, Li Jun, Wang Meiwei, Wang Qiye, Li Jianzhong, Ding Nengshui, Yang Huansheng, Yin Yulong
机构信息
Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
出版信息
Anim Nutr. 2021 Dec;7(4):1070-1077. doi: 10.1016/j.aninu.2021.05.002. Epub 2021 Sep 8.
The present study aimed to investigate whether inflammation-associated responses in piglets are induced by high protein (HP) through activating nuclear factor kappa B (NF-κB) signaling. Sixteen piglets (35 d of age, Duroc × [Landrace × Yorkshire], weaned at d 21, initial BW = 9.70 ± 0.11 kg) were allocated to 18% and 26% CP (HP group) at random, comprising 8 replicate pens per treatment. The piglets were slaughtered to collect intestinal tissues when apparent, persistent, and stable diarrhea syndromes happened (on d 12). No significant differences were observed in their growth performance ( > 0.05), but reduction by 19.11%, 25.31%, 23.64% of ADFI, ADG, and G:F, respectively was detected in the HP group. The HP group had greater ( = 0.002) diarrhea rates. Furthermore, dietary HP had lower ileal villus height (VH; = 0.048), ratio of villus height to crypt depth (VH/CD ratio; = 0.016), and colonic CD ( = 0.034), as well as had the trend ( = 0.075) to reduce the ileal villus absorptive area. Moreover, HP diets significantly elevated the goblet cell numbers in the ileal villi ( = 0.016) and colonic crypts ( < 0.001) and up-regulated ( = 0.012) the mRNA expression of mucin2 () in the ileum. In addition, HP diets increased the myeloperoxidase concentration in the ileum ( = 0.002) and colon ( = 0.007) of piglets. Dietary HP significantly down-regulated the mRNA expression of tumor necrosis factor-α (; < 0.001) in the ileum, induced nitric oxide synthase (; = 0.040) and interleukin-22 (; = 0.008) in the colon, and inclined to down-regulate interleukin-1β (; = 0.076) expression in the colon. The relative protein abundance of Galectin-3 ( = 0.046) in the colon and the ratio of phosphorylation NF-κB to NF-κB (p-NF-κB/NF-κB ratio) in the ileum of HP piglets were also greater ( = 0.038). These results suggest that dietary HP may cause diarrhea in piglets by activating NF-κB signaling induced intestinal inflammation.
本研究旨在探讨高蛋白（HP）是否通过激活核因子κB（NF-κB）信号通路诱导仔猪炎症相关反应。16头仔猪（35日龄，杜洛克×[长白×约克夏]，21日龄断奶，初始体重=9.70±0.11千克）随机分为18%和26%粗蛋白组（HP组），每组8个重复栏。当出现明显、持续且稳定的腹泻综合征时（第12天）宰杀仔猪以收集肠道组织。它们的生长性能未观察到显著差异（P>0.05），但HP组的平均日采食量（ADFI）、平均日增重（ADG）和料重比（G:F）分别降低了19.11%、25.31%和23.64%。HP组腹泻率更高（P=0.002）。此外，日粮HP降低了回肠绒毛高度（VH；P=0.048）、绒毛高度与隐窝深度之比（VH/CD比；P=0.016）和结肠隐窝深度（P=0.034），并且有降低回肠绒毛吸收面积的趋势（P=0.075）。此外，HP日粮显著增加了回肠绒毛和结肠隐窝中的杯状细胞数量（P=0.016和P<0.001），并上调了回肠中粘蛋白2（MUC2）的mRNA表达（P=0.012）。另外，HP日粮增加了仔猪回肠（P=0.002）和结肠（P=0.007）中的髓过氧化物酶浓度。日粮HP显著下调了回肠中肿瘤坏死因子-α（TNF-α；P<0.001）的mRNA表达，诱导了结肠中一氧化氮合酶（iNOS；P=0.040）和白细胞介素-22（IL-22；P=0.008）的表达，并倾向于下调结肠中白细胞介素-1β（IL-1β；P=0.076）的表达。HP组仔猪结肠中半乳糖凝集素-3的相对蛋白丰度（P=0.046）以及回肠中磷酸化NF-κB与NF-κB的比值（p-NF-κB/NF-κB比值）也更高（P=0.038）。这些结果表明，日粮HP可能通过激活NF-κB信号通路诱导肠道炎症，从而导致仔猪腹泻。
Zotero 插件