Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Respir Physiol Neurobiol. 2012 Jan 15;180(1):141-6. doi: 10.1016/j.resp.2011.10.018. Epub 2011 Nov 4.
A new intravascular fluorosensor was developed and validated for inline P(CO)₂monitoring. The P(CO)₂sensor was based on the fluorescent indicator 1-hydroxypyrene-3,6,8-trisulfonate. The P(CO)₂sensor was then immersed in various solutions in vitro and carotid artery bypass of rabbits in vivo for testing. Changes of P(CO)₂in solutions and blood were created by bubbling CO2/N2 and hyperventilation/hypoventilation, respectively. The changes of fluorescent intensity over P(CO)₂ range of 14-150 mmHg was linear. The resolution of the whole sensor system was 1 mmHg, with a bias ± SD of -0.1 ± 2.9 mmHg and precision ± SD of 2.1 ± 1.9 mmHg. The sensor signal has been stable during measurement for at least 25h and was insensitive to fluctuations of ions concentration and osmosis at pathophysiological limits. The performance of the sensor is in agreement with blood gas analyzer in a wide range of P(CO)₂and it is qualified for continuous intravascular measurement of blood P(CO)₂at various conditions.
一种新的血管内荧光传感器被开发并验证用于在线监测 P(CO)₂。该 P(CO)₂传感器基于荧光指示剂 1-羟基芘-3,6,8-三磺酸。然后，该 P(CO)₂传感器在体外的各种溶液中和体内的兔颈动脉旁路中进行了测试。通过向 CO2/N2 吹气和过度通气/通气不足，分别在溶液和血液中产生 P(CO)₂的变化。荧光强度随 P(CO)₂范围从 14 到 150mmHg 的变化呈线性。整个传感器系统的分辨率为 1mmHg，偏差为±2.9mmHg，精度为±1.9mmHg。在测量过程中，传感器信号至少稳定 25 小时，并且对病理生理极限下的离子浓度和渗透压波动不敏感。传感器的性能在广泛的 P(CO)₂范围内与血气分析仪一致，并且有资格在各种条件下进行连续的血管内血液 P(CO)₂测量。
