Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
IEEE Trans Biomed Eng. 2011 May;58(5):1232-8. doi: 10.1109/TBME.2011.2107514. Epub 2011 Jan 20.
Continuous intra-arterial blood pH monitoring is highly desirable in clinical practice. However, devices with appreciable accuracy are still not commercially available to date. In this study, we present a fiber-optic fluorosensor that can be used to continuously and accurately measure blood pH changes. The pH sensor is developed based on a proton-sensitive fluorescence dye, N-allyl-4-(4'-methyl-piperazinyl)-1,8-naphthalimide, which is bonded covalently to an optical fiber through heat polymerization. Fluorescence intensity was recorded after the sensor was exposed to different pH buffer solutions or intra-arterial blood in rabbits. Fluorescence intensity with emission peak at 510 nm decreased immediately as the blood pH increased. Linear and reproducible responses were observed when pH ranges from 6.8 to 8.0 with resolution of 0.03 pH units. The correlation coefficient between the pH sensor and the conventional blood gas analyzer was 0.93 in vivo ( n=75, p<0.001) with a bias and precision of -0.02 ± 0.08 pH units. The pH sensor was stable during measurement for at least 72 h. The pH sensor is not sensitive to fluctuations of various ions' concentrations and plasma osmosis at pathophysiological limits, suggesting that it is useful for the continuous measurement of blood pH at various clinical settings.
在临床实践中，连续的动脉内血液 pH 值监测是非常理想的。然而，到目前为止，还没有具有相当准确性的设备可供商业使用。在这项研究中，我们提出了一种光纤荧光传感器，可用于连续、准确地测量血液 pH 值变化。该 pH 值传感器是基于质子敏感荧光染料 N-丙烯基-4-(4'-甲基-哌嗪基)-1,8-萘酰亚胺开发的，该染料通过热聚合共价键合到光纤上。在传感器暴露于不同的 pH 缓冲溶液或兔子的动脉内血液后，记录荧光强度。当血液 pH 值增加时，发射峰在 510nm 的荧光强度立即降低。当 pH 值范围在 6.8 到 8.0 时，观察到线性和可重复的响应，分辨率为 0.03 pH 单位。在体内（n=75，p<0.001），pH 值传感器与常规血气分析仪之间的相关系数为 0.93，偏差和精度为-0.02±0.08 pH 单位。在测量过程中，pH 值传感器至少稳定 72 小时。该 pH 值传感器对各种离子浓度和病理生理极限下的血浆渗透压波动不敏感，表明它可用于各种临床环境下的血液 pH 值连续测量。
