Laboratory of Biomechanics and Biocalorimetry, LOB2, c/o Biozentrum/Pharmazentrum, University of Basel, Basel, Switzerland.
J Clin Microbiol. 2012 Jan;50(1):16-20. doi: 10.1128/JCM.05556-11. Epub 2011 Nov 16.
Mycobacterium tuberculosis is a global public health concern, particularly with the emergence of drug-resistant strains. Immediate identification of drug-resistant strains is crucial to administering appropriate treatment before the bacteria are allowed to spread. However, developing countries, which are most affected by drug resistance, are struggling to combat the disease without the facilities or funds for expensive diagnostics. Recent studies have emphasized the suitability of isothermal microcalorimetry (IMC) for the rapid detection of mycobacteria. In this study, we investigate its suitability for rapid and reliable M. tuberculosis drug susceptibility testing. Specifically, IMC was used to determine the MICs of three drugs, namely, isoniazid, ethambutol, and moxifloxacin, against three mycobacteria, namely, Mycobacterium smegmatis, Mycobacterium avium, and Mycobacterium tuberculosis. The Richards growth model was used to calculate growth parameters, namely, the maximum bacterial growth rate and the lag phase duration from integrated heat flow-versus-time results. For example, MICs of isoniazid, ethambutol, and moxifloxacin were determined to be 1.00, 8.00, and 0.25 μg/ml, respectively. IMC, as described here, could be used not just in industrialized countries but also in developing countries because inexpensive and sensitive microcalorimeters are now available.
结核分枝杆菌是全球公共卫生关注的焦点，特别是出现耐药菌株的情况下。在细菌传播之前，立即识别耐药菌株对于给予适当的治疗至关重要。然而，受耐药性影响最大的发展中国家在没有昂贵诊断设备或资金的情况下，正在努力对抗这种疾病。最近的研究强调了等温微量量热法（IMC）在快速检测分枝杆菌方面的适用性。在这项研究中，我们研究了它在快速可靠地检测结核分枝杆菌药物敏感性方面的适用性。具体来说，我们使用 IMC 来确定三种药物（异烟肼、乙胺丁醇和莫西沙星）对三种分枝杆菌（耻垢分枝杆菌、鸟分枝杆菌和结核分枝杆菌）的最低抑菌浓度（MIC）。使用 Richards 生长模型从整合热流与时间的结果中计算生长参数，即最大细菌生长率和迟滞期持续时间。例如，异烟肼、乙胺丁醇和莫西沙星的 MIC 分别确定为 1.00、8.00 和 0.25 μg/ml。如这里所述，IMC 不仅可以在工业化国家使用，也可以在发展中国家使用，因为现在已经有了价格低廉且敏感的微量量热计。
