Department of Surgery, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, California 90095-7098, USA.
Surg Obes Relat Dis. 2013 Jan-Feb;9(1):108-12. doi: 10.1016/j.soard.2011.09.025. Epub 2011 Oct 14.
Although women disproportionately undergo bariatric surgery, the rodent models investigating the mechanisms of bariatric surgery have been limited to males. Female rodent models can also potentially allow us to understand the effects of surgical intervention on future generations of offspring. Sleeve gastrectomy is an attractive weight loss procedure for reproductive-age female patients because it avoids the malabsorption associated with intestinal bypass. We sought to evaluate the effect of sleeve gastrectomy on young female rats with diet-induced obesity at the University of California, Los Angeles, David Geffen School of Medicine.
Sprague-Dawley female rats were fed a 60% high-fat diet. At 12 weeks of age, the rats underwent either sleeve gastrectomy or sham surgery. The rats were killed 4 weeks after surgery. A chemistry panel was performed, and the serum adipokines and gut hormones were assayed. The homeostasis model assessment score was calculated. The liver histologic findings were graded for steatosis. The 2-sample t test was used to compare the results between the 2 groups.
Sleeve gastrectomy was associated with significant weight loss (5% ± 6% versus -4% ± 6%; P < .001), lower leptin levels (1.3 ± 1.2 versus 3.5 ± 2.3 ng/mL; P < .01), and higher adiponectin levels (.43 ± .19 versus .17 ± .14 ng/mL; P < .004) compared with the sham-operated rats. No significant differences were found in the fasting ghrelin levels. Furthermore, we did not observe evidence of insulin resistance or steatohepatitis after 11 weeks of high-fat diet. Despite these limitations, additional gender-specific studies are warranted given that most bariatric surgeries are performed in women.
Sleeve gastrectomy appears to result in weight loss and improvements in adiponectin and leptin by way of mechanisms independent of ghrelin levels in a female model of diet-induced obesity.
尽管女性接受减重手术的比例不成比例，但研究减重手术机制的啮齿动物模型仅限于男性。女性啮齿动物模型还可以帮助我们了解手术干预对后代的影响。袖状胃切除术是一种有吸引力的减肥手术，适用于生殖年龄的女性患者，因为它避免了与肠旁路相关的吸收不良。我们试图在加利福尼亚大学洛杉矶分校大卫格芬医学院评估袖状胃切除术对患有饮食诱导肥胖的年轻雌性大鼠的影响。
Sprague-Dawley 雌性大鼠喂食 60%高脂肪饮食。在 12 周龄时，大鼠接受袖状胃切除术或假手术。手术后 4 周处死大鼠。进行化学面板检查，并测定血清脂肪因子和肠道激素。计算稳态模型评估评分。肝脏组织学发现为脂肪变性。使用两样本 t 检验比较两组结果。
与假手术组相比，袖状胃切除术与显著的体重减轻（5%±6%对-4%±6%；P<0.001）、较低的瘦素水平（1.3±1.2 对 3.5±2.3ng/mL；P<0.01）和较高的脂联素水平（0.43±0.19 对 0.17±0.14ng/mL；P<0.004）相关。空腹胃饥饿素水平无显著差异。此外，在 11 周高脂肪饮食后，我们没有观察到胰岛素抵抗或脂肪性肝炎的证据。尽管存在这些局限性，但鉴于大多数减重手术都是在女性中进行的，因此需要进行更多的性别特异性研究。
在饮食诱导肥胖的雌性模型中，袖状胃切除术似乎通过独立于胃饥饿素水平的机制导致体重减轻和脂联素和瘦素水平的改善。
