School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Talanta. 2011 Dec 15;87:302-6. doi: 10.1016/j.talanta.2011.09.049. Epub 2011 Oct 1.
A novel magnetic electrochemical immunosensor has been developed for the detection of pseudorabies virus antibody in swine serum. The magnetic glass carbon electrode was fabricated to manipulate magnetic beads for the direct sensing applications. Magnetic beads were employed as the platforms for the immobilization and immunoreaction process, and gold nanoparticles were chosen as electroactive labels for the electrochemical detection. The parameters concerning the assay strategy were carefully investigated. Under the optimal conditions, the linear response range of pseudorabies virus antibody dilution ratio (standard positive serum) was 1:250 to 1:1000 with a detection limit of 1:1000. Finally, this developed immunoassay method was successfully applied in the detection of pseudorabies virus antibody in swine serum, and had a good diagnostic accordance in comparison with ELISA.
一种新型的磁电化学免疫传感器已被开发用于检测猪血清中的伪狂犬病病毒抗体。磁性玻碳电极被用于操作磁珠，以实现直接传感应用。磁珠被用作固定化和免疫反应过程的平台，金纳米粒子被选择作为电化学检测的电活性标记。仔细研究了与分析策略相关的参数。在最佳条件下，伪狂犬病病毒抗体稀释比（标准阳性血清）的线性响应范围为 1:250 至 1:1000，检测限为 1:1000。最后，该免疫分析方法成功应用于猪血清中伪狂犬病病毒抗体的检测，与 ELISA 相比具有良好的诊断一致性。
