Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Vet Microbiol. 2012 Apr 23;156(1-2):217-21. doi: 10.1016/j.vetmic.2011.10.023. Epub 2011 Nov 3.
In this study, we have successfully used molecular methods based on the amplification of the 16S ribosomal RNA gene on feline bile samples to show that bile of cats with LC is not sterile. This is probably due to the fact that the inflammatory process in the biliary tree causes dilatations. As a result, bacteria can easily migrate from the intestines via the common bile duct. The diversity of species identified and the presence of Helicobacter spp. DNA in both patients and controls suggests that bacteriobilia is secondary to the disease and is not the cause of LC.
在这项研究中，我们成功地使用了基于猫胆汁样本中 16S 核糖体 RNA 基因扩增的分子方法，证明了患有 LC 的猫的胆汁并非无菌。这可能是由于胆道炎症过程导致胆管扩张，结果，细菌可以很容易地从肠道经胆总管迁移。在患者和对照组中鉴定出的物种多样性和幽门螺旋杆菌 DNA 的存在表明，胆菌血症是继发于疾病的，而不是 LC 的原因。
