在大肠杆菌的细胞分裂过程中，细胞质和周质依次闭合。
Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli.
机构信息
Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
出版信息
J Bacteriol. 2012 Feb;194(3):584-6. doi: 10.1128/JB.06091-11. Epub 2011 Nov 18.
Abstract
To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event.
摘要
为了在活的大肠杆菌中可视化细胞分裂的后期阶段，我们对 121 个表达细胞质绿色荧光蛋白和周质 mCherry 的细胞进行了光漂白后荧光恢复（FRAP）实验。我们的数据明确表明，细胞质在分裂事件中先于周质被密封。
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