日本金龟子芽孢杆菌和缓死芽孢杆菌KLN2的GATC序列中N6-甲基腺嘌呤的存在情况。
Presence of N6-methyladenine in GATC sequences of Bacillus popilliae and Bacillus lentimorbus KLN2.
作者信息
Dingman D W
机构信息
Connecticut Agricultural Experiment Station, New Haven 06504.
出版信息
J Bacteriol. 1990 Oct;172(10):6156-9. doi: 10.1128/jb.172.10.6156-6159.1990.
Abstract
Nine strains of Bacillus popilliae and Bacillus lentimorbus KLN2 contain N6-methyladenine in GATC sequences, as determined by using the restriction enzymes MboI and DpnI. Among eight other Bacillus species examined, all, except one strain of Bacillus brevis (ATCC 9999), lacked adenine methylation in GATC. A methylase with Escherichia coli dcm site specificity was not present in any of the Bacillus species studied.
摘要
通过使用限制性内切酶MboI和DpnI测定，9株日本金龟子芽孢杆菌和缓死芽孢杆菌KLN2在GATC序列中含有N6-甲基腺嘌呤。在检测的其他8种芽孢杆菌中，除了短短芽孢杆菌的一个菌株（ATCC 9999）外，所有菌株在GATC中都缺乏腺嘌呤甲基化。在所研究的任何芽孢杆菌物种中都不存在具有大肠杆菌dcm位点特异性的甲基化酶。
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