Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.
J Mater Sci Mater Med. 2012 Feb;23(2):485-95. doi: 10.1007/s10856-011-4507-0. Epub 2011 Nov 30.
This study aimed at preparation and in vitro and in vivo evaluation of novel bioactive, biodegradable, and antibacterial nanocomposite coating for the improvement of stem cells attachment and antibacterial activity as a candidate for dental implant applications. Poly (lactide-co-glycolide)/bioactive glass/hydroxyapatite (PBGHA) nanocomposite coating was prepared via solvent casting process. The nanoparticle amounts of 10, 15, and 20 weight percent (wt%) were chosen in order to determine the optimum amount of nanoparticles suitable for preparing an uniform coating. Bioactivity and degradation of the coating with an optimum amount of nanoparticles were evaluated by immersing the prepared samples in simulated body fluid and phosphate buffer saline (PBS), respectively. The effect of nanocomposite coating on the attachment and viability of human adipose-derived stem cells (hASCs) was investigated. Kirschner wires (K-wires) of stainless steel were coated with the PBGHA nanocomposite coating, and mechanical stability of the coating was studied during intramedullary implantation into rabbit tibiae. The results showed that using 10 wt% nanoparticles (5 wt% HA and 5 wt% BG) in the nanocomposite could provide the desired uniform coating. The study of in vitro bioactivity showed rapid formation of bone-like apatite on the PBGHA coating. It was degraded considerably after about 60 days of immersion in PBS. The hASCs showed excellent attachment and viability on the coating. PBGHA coating remained stable on the K-wires with a minimum of 96% of the original coating mass. It was concluded that PBGHA nanocomposite coating provides an ideal surface for the stem cells attachment and viability. In addition, it could induce antibacterial activity, simultaneously.
本研究旨在制备新型生物活性、可生物降解和抗菌的纳米复合涂层，以提高干细胞的附着和抗菌活性，作为牙科植入物应用的候选材料。采用溶剂浇铸法制备聚（丙交酯-乙交酯）/生物活性玻璃/羟基磷灰石（PBGHA）纳米复合涂层。选择纳米粒子含量为 10、15 和 20 重量%（wt%），以确定适合制备均匀涂层的最佳纳米粒子用量。通过将制备的样品分别浸入模拟体液和磷酸盐缓冲盐水（PBS）中，评估涂层的生物活性和降解情况。研究了纳米复合涂层对人脂肪源性干细胞（hASCs）附着和活力的影响。不锈钢克氏针（K-wires）涂有 PBGHA 纳米复合涂层，并研究了涂层在髓内植入兔胫骨过程中的机械稳定性。结果表明，在纳米复合材料中使用 10wt%的纳米粒子（5wt%HA 和 5wt%BG）可以提供所需的均匀涂层。体外生物活性研究表明，PBGHA 涂层上迅速形成类骨磷灰石。在 PBS 中浸泡约 60 天后，涂层会大量降解。hASCs 在涂层上具有良好的附着和活力。PBGHA 涂层在 K-wires 上保持稳定，原始涂层质量的最小损失为 96%。结论是 PBGHA 纳米复合涂层为干细胞的附着和活力提供了理想的表面。此外，它还可以同时诱导抗菌活性。
