Tandogan Berivan, Güvenç Ayşegül, Çalış İhsan, Ulusu Nuriye Nuray
Department of Biochemistry, Hacettepe University, Ankara, Turkey.
Acta Biochim Pol. 2011;58(4):471-5. Epub 2011 Dec 6.
Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.
谷胱甘肽还原酶（GR，E.C 1.6.4.2）是一种黄素蛋白，可催化依赖NADPH的氧化型谷胱甘肽（GSSG）还原为还原型谷胱甘肽（GSH）。本研究的目的是调查从短苞铁角蕨中分离出的酚类化合物对牛肾GR的体外作用。铁角蕨属植物在自然界中广泛存在，通常用作药用植物。从短苞铁角蕨地上部分分离出了8-羟基黄酮的7-O-糖苷（异槲皮苷、异黄芩素和3'-羟基-4'-O-甲基异黄芩素）。这些化合物以浓度依赖性方式抑制牛肾皮质GR。还对抑制作用进行了动力学表征。
