School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
J Am Chem Soc. 2012 Jan 11;134(1):119-22. doi: 10.1021/ja209676p. Epub 2011 Dec 19.
Polypeptide block copolymers with different block length ratios were obtained by sequential ring-opening polymerization of benzyl-L-glutamate and propargylglycine (PG) N-carboxyanhydrides. Glycosylation of the poly(PG) block was obtained by Huisgens cycloaddition "click" reaction using azide-functionalized galactose. All copolymers were self-assembled using the nanoprecipitation method to obtain spherical and wormlike micelles as well as polymersomes depending on the block length ratio and the nanoprecipitation conditions. These structures display bioactive galactose units in the polymersome shell, as proven by selective lectin binding experiments.
采用苄基-L-谷氨酸和丙炔甘氨酸（PG）N-羧酸酐的顺序开环聚合反应得到具有不同嵌段长度比的多肽嵌段共聚物。通过使用叠氮基功能化半乳糖的 Huisgens 环加成“点击”反应得到聚（PG）嵌段的糖基化。所有共聚物均通过纳米沉淀法自组装，根据嵌段长度比和纳米沉淀条件获得球形和蠕虫状胶束以及聚合物囊泡。这些结构在聚合物囊泡壳中显示出具有生物活性的半乳糖单元，这通过选择性凝集素结合实验得到证明。
