Walczak Anna, Przybyłowska Karolina, Trzciński Radzisław, Sygut Andrzej, Dziki Lukasz, Dziki Adam, Majsterek Ireneusz
Department of Clinical Chemistry and Biochemistry, Medical University in Łódź.
Pol Przegl Chir. 2011 Jan;83(1):27-31. doi: 10.2478/v10035-011-0004-x.
Colorectal carcinoma is one of the leading causes of death from cancer amongst adults. Considering its molecular background, cytokines are the key component of the inflammatory microenvironment of these tumors. Investigations that enable better understanding of colorectal cancer concerning the molecular level, may provide important tools for genetic screening of disease high-risk groups, as well as molecular diagnostics for the non-invasive detection of cancer in its early stages.THE AIM OF THE STUDY was to evaluate the association between colorectal cancer and the -1112 C/T single nucleotide polymorphism (SNP) of the interleukin-13 gene. MATERIAL AND METHODS. The study group comprised 150 cancer patients and 170 healthy subject genotypes from the Polish population. Analysis was performed by PCR-restriction fragment length polymorphism (PCR-RFLP). RESULTS. We showed that the CT genotype is connected with a higher risk of colon cancer occurrence (OR 2.51; 95% CI 1.57-4.02; p < 0.0001). We also correlated the polymorphic variants of the IL-13 gene with the clinical characteristics of colorectal cancer patients. We observed no association between the investigated polymorphism and colorectal cancer progression, evaluated by tumor stage, as well as lymph node metastasis. CONCLUSIONS. The presented study suggested the possibility of a connection between the IL-13 gene polymorphism (-1112 C/T) and colorectal cancer risk in the Polish population.
结直肠癌是成年人癌症死亡的主要原因之一。考虑到其分子背景，细胞因子是这些肿瘤炎症微环境的关键组成部分。能够在分子水平上更好地理解结直肠癌的研究，可能为疾病高危人群的基因筛查以及癌症早期非侵入性检测的分子诊断提供重要工具。本研究的目的是评估结直肠癌与白细胞介素-13基因-1112 C/T单核苷酸多态性（SNP）之间的关联。材料与方法。研究组包括150名来自波兰人群的癌症患者和170名健康受试者的基因型。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行分析。结果。我们发现CT基因型与结肠癌发生风险较高相关（比值比2.51；95%置信区间1.57 - 4.02；p < 0.0001）。我们还将白细胞介素-13基因的多态性变体与结直肠癌患者的临床特征相关联。通过肿瘤分期以及淋巴结转移评估，我们未观察到所研究的多态性与结直肠癌进展之间存在关联。结论。本研究表明波兰人群中白细胞介素-13基因多态性（-1112 C/T）与结直肠癌风险之间可能存在联系。
